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Clone: HB17N02

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2019
  tissue: developing caryopsis
  stage/tissue: 8-15 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
 
Sequence: HB17N02r
submitter of sequence: Dr. Nils Stein
length: 379
priming: 5-prime
GenBankAc: BU971503
IPK CR-EST: HB17N02r 
HB17N02r
CGGCACGAGG CTCCAGTGTT GGCGCTCAGA AAGTGAAGCT CGCCTGCATG CACTTCCGCC         60
AGTTCTATGA GGCAAAAAGT AAAGATGGNT GCCTCATGGC GTTGGCCCTT GTTAGGAGTG        120
AGTTCTGTGA TGTGCGCAGC AAGTTCCAGA CCATGATGCA GCTGGGGCAG CAGATCGAGG        180
CCTGCAGTCC CAAGTAGAGT AGCAGTAAAA ACTGAAGCAG AAGGAGCTTA NCTCCATGTG        240
ACCCCTGACA TGATGGTGGG GGGGCAGTGT GTATGTATCC CTTAAACTTG TTTGTAGGCA        300
GTGTAACGGC TGTGTGTGTG CAGAACCGGA TTGACCGATT CCAGCGGTGG AACTCCGGGC        360
TCGCGACCCC TTGAAACGG                                                     379
TypePositionOrientation
ORF 2 - 67: forward
ORF 3 - 194: forward
ORF 67 - 153: forward
ORF 273 - 371: forward
ORF 377 - 285: reverse
ORF 378 - 115: reverse
ORF 379 - 68: reverse
Cluster Info:
Contig3529.1 (9 members)
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