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Clone: HA21B12
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species: |
Hordeum vulgare |
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type: |
wildtype |
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common name: |
barley |
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cultivar: |
Barke |
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lib name: |
IPKc 2018 |
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tissue: |
embryosac |
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stage/tissue: |
0-7 DAP (days after pollination) |
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cloning vector: |
pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA) |
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host: |
XL10-Gold |
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antibiotic: |
Ampicillin |
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library description: |
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0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
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| Sequence features and Clone alignments (BLAST): |
 UTR |
 start codon |
 stop codon |
 exon-exon border |
 forward |
 reverse |
 start |
 stop |
 domain |
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| Click on sequence features to obtain additional information. | |
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HA21B12r
CGGCACGAGG CGTCGATGGT AGAAGGTCGC GCGGTGGTGG TGCCGGCGGC GGACTGCTCG 60
GTGACTGTGA TCGGGCGGGG CGAGAAGTGC GAGCCCCGGA AGTGCGAGGC GGAGTGTCGG 120
AGGCAGCACG ATGGTATGGG CGCGTGCGCC GCCATGGGGT GCAGTTGCGC CGTCTGCGCC 180
AAGTATCCTC CCTCCTCCTC CGTCGCAAAG GGAAACCGAT ACTGATAAAT GACGACTAGT 240
AGTAGTACAC CAGTGCTAGG TTCAAAGTGT GTACTCACTA GATAGCGGGG ATCCTTGCAA 300
TGCTCATTTT CACAGATAAT TTGGATCTGA TAAACAATAT GTAGTACATG AATGCTTGAC 360
AACAAGAAAT AATCCTTGAT GATTAACTCG TGGT 394
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| ORF |
1 - 222: |
forward |
| ORF |
3 - 236: |
forward |
| ORF |
131 - 229: |
forward |
| ORF |
155 - 3: |
reverse |
| ORF |
349 - 281: |
reverse |
| ORF |
393 - 313: |
reverse |
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GabiPD Webmaster |
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