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Clone: HA03E02
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species: |
Hordeum vulgare |
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type: |
wildtype |
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common name: |
barley |
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cultivar: |
Barke |
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lib name: |
IPKc 2018 |
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tissue: |
embryosac |
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stage/tissue: |
0-7 DAP (days after pollination) |
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cloning vector: |
pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA) |
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host: |
XL10-Gold |
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antibiotic: |
Ampicillin |
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library description: |
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0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
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| Sequence features and Clone alignments (BLAST): |
 UTR |
 start codon |
 stop codon |
 exon-exon border |
 forward |
 reverse |
 start |
 stop |
 domain |
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| Click on sequence features to obtain additional information. | |
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HA03E02u
AATCATCAAG GATTATTTCT TGTTGTCAAG CATTCATGTA CTACATATTG TTTATCAGAT 60
CCAAATTATC TGTGAAAATG AGCATTGCAA GGATCCCCGC TATCTAGGGA GTACACACTT 120
TGAACCTAGC ACTGGGGTAC TACTACTAGT CGTCATTTAT CAGTATCGGT TTCCCTTTGC 180
GACGGAGGAG GAGGGAGGAT ACTTGGCGCA AACGGCGCAA CTGCACCCCA TGGCGGCGCA 240
CGCGCCCATA CCATCGTGCT GCCTCCGACA CTCCGCCTCG CATTTCCGGG GCTCGCACTT 300
CTCGCCCCGC CCGATCACAG TCACCGAGCA GTCCGCCGCC GGCACCACCA CCGGGCGACC 360
TTTTACCATC GACGGTGTGT ATGTGCTTGA TGATGACAGC ACCACGAGAG CGAGGGACAC 420
CAGGAGCAGC AGGGCGTT 438
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| ORF |
1 - 72: |
forward |
| ORF |
3 - 104: |
forward |
| ORF |
230 - 436: |
forward |
| ORF |
254 - 156: |
reverse |
| ORF |
436 - 149: |
reverse |
| ORF |
437 - 366: |
reverse |
| ORF |
438 - 163: |
reverse |
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GabiPD Webmaster |
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