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Clone: HA02G05

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb
 
Sequence: HA02G05r
submitter of sequence: Dr. Nils Stein
length: 526
priming: 5-prime
GenBankAc: BQ459074
IPK CR-EST: HA02G05r 
HA02G05r
CGGCACGAGG CATCGTTCCA AAATTCAATC CAGGAGAACA TGTCAACAAG AGAGGTGAAC         60
CAGGAGGCGA GGTAGAGTAA CAGGCTATGG AGCTACTATT GTACCTCGCC TTGTCCAAGC        120
AACTTCATGG AGGCAATTAT TTCCTCTTTC ATCCTTATAG ATTCCTAGTT TCCTATCCAG        180
GGGAACTTCT CCGATCACCA TCCACAACAC AAGCAGCAAA TATTTGTAGC TAGGCACACA        240
TCAAGGATAT TGAATGATGT CTAGAGCATA ATGACCAAGA TGAAGCGCTA CACTTTGTCG        300
AGCGGATTCC TTGTTGGTAC AAACTAGAAA ATATGCAGAG CTCTTCAACA ACATTGATAC        360
AATGCTACTA GATTGGACTG AAAATAATGG TGTACGCTTT TTTCTTGTTT TTTGTTGCAA        420
AAAGAATTGT CAGTGGCCAT TTTTTGTACT TTGCGGAACA TATGTCATAA TTGTAACAGG        480
GTTACAATTT TTCACTTTTG ATGATAACAA ATTGCTATTT GGTTGG                       526
TypePositionOrientation
ORF 1 - 72: forward
ORF 3 - 77: forward
ORF 87 - 230: forward
ORF 201 - 88: reverse
ORF 354 - 244: reverse
ORF 362 - 526: forward
ORF 387 - 467: forward
ORF 461 - 348: reverse
ORF 525 - 433: reverse
Cluster Info:
Contig19762.1 (2 members)
Representative for Uniset
view Blast Searches
 
Sequence: RUS01A10w
submitter of sequence: Dr. Nese Sreenivasulu
length: 487
priming: 3-prime
GenBankAc: FD521430
IPK CR-EST: RUS01A10w 
RUS01A10w
CCAAATAGCA ATTTGTTATC ATCAAAAGTG AAAAATTGTA ACCCTGTTAC AATTATGACA         60
TATGTTCCGC AAAGTACAAA AAATGGCCAC TGACAATTCT TTTTGCAACA AAAAACAAGA        120
AAAAAGCGTA CACCATTATT TTCAGTCCAA TCTAGTAGCA TTGTATCAAT GTTGTTGAAG        180
AGCTCTGCAT ATTTTCTAGT TTGTACCAAC AAGGAATCCG CTCGACAAAG TGTAGCGCTT        240
CATCTTGGTC ATTATGCTCT AGACATCATT CAATATCCTT GATGTGTGCC TAGCTACAAA        300
TATTTGCTGC TTGTGTTGTG GATGGTGATC GGAGAAGTTC CCCTGGATAG GAAACTAGGA        360
ATCTATAAGG ATGAAAGAGG AAATAATTGC CTCCATGAAG TTGCTTGGAC AAGGCGAGGT        420
ACAATAGTAG CTCCATAGCC TGTTACTCTA CCTCGCCTCC TGGTTCACCT CTCTTGTTGA        480
CATGTTC                                                                  487
TypePositionOrientation
ORF 1 - 90: forward
ORF 62 - 175: forward
ORF 136 - 56: reverse
ORF 161 - 3: reverse
ORF 169 - 279: forward
ORF 322 - 435: forward
ORF 436 - 293: reverse
Cluster Info:
Contig19762.1 (2 members)
view Blast Searches
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