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Clone: HA01A13

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
Hv.29457 unigene cluster
 
Sequence: HA01A13r
submitter of sequence: Dr. Nils Stein
length: 146
priming: 5-prime
GenBankAc: BQ459459
IPK CR-EST: HA01A13r 
HA01A13r
CGGCACGAGG CTCGCGGCGC GTGCTCTTCC GTTCCATGGG GTGATGGATG AGATCCTGCG         60
AGCTGTTGCT GGATCGAGCC TGATCGCCAC GACAATGTGG AACTGCCCTG CGCACTCCTC        120
GCTCCTGCTG CCCATGGGCG ACGACC                                             146
TypePositionOrientation
ORF 44 - 145: forward
ORF 135 - 1: reverse
Singlet Info:
AA tgicl singlet
Representative for Uniset
view Blast Searches
 
Sequence: HA01A13u
submitter of sequence: Dr. Nils Stein
length: 395
priming: 3-prime
GenBankAc: BQ657024
IPK CR-EST: HA01A13u 
HA01A13u
ATCGGGAGCA TGAAACTATA ACCTGTATTT ATCCATGGAA ATCAGACATT CATAGGAGCT         60
TAACATCCAT TCCCTTTCAC CGAGTGGACG TGAGCACTGA TCGAGCTAAC ACGCCATGTG        120
AATGAGTCAG ATACGAGCAG CAAATTATTC TAGGTTTCTA CAGTACTGTA GCTAGCATCG        180
CAGTAGAAGG ACATGCCATG GTGACGATTA TATGGTCGTG TCCTGTACCG TTCTTCTACA        240
TAGACTCTAA CCAACGTAAC CTTCTTCAGC TGAGCTACAG AGCAGTGAAC CAAGCACCCC        300
CCACGTCGGC GTGAGCGCGG CCGTGGTCAC GCGCTGCGCC GGACACGAGG CTCGCGACGG        360
CCGGCGAAAG CGGGGCCACG CATAGGCCAC GCCGG                                   395
TypePositionOrientation
ORF 3 - 122: forward
ORF 198 - 311: forward
ORF 393 - 250: reverse
ORF 394 - 278: reverse
ORF 395 - 330: reverse
Cluster Info:
Contig17200.1 (2 members)
view Blast Searches
 
Sequence: RUS01E05w
submitter of sequence: Dr. Nese Sreenivasulu
length: 697
priming: 3-prime
GenBankAc: FD521468
IPK CR-EST: RUS01E05w 
RUS01E05w
ATCGTGAGCA TGAAACTATA ACCTGTATTT ATCCATGGAA ATCAGACATT CATAGGAGCT         60
TAACATCCAT TCCCTTTCAC CGAGTGGACG TGAGCACTGA TCGAGCTAAC ACGCCATGTG        120
AATGAGTCAG ATACGAGCAG CAAATTATTC TAGGTTTCTA CAGTACTGTA GCTAGCATCG        180
CAGTAGAAGG ACATGCCATG GTGACGATTA TATGGTCGTG TCCTGTACCG TTCTTCTACA        240
TAGACTCTAA CCAACGTAAC CTTCTTCAGC TGAGCTACAG AGCAGTGAAC CAAGCACCCC        300
CCACGTCGGC GTGAGCGCGG CCGTGGTCAC GCGCTGCGCC GGACACGAGG CTCGCGACGG        360
CCGGCGAAAG CGGGGCCACG CATAGGCCAC GCCGGCGCAG GCCCGTCGCG GGCTCCTCTC        420
CGGCATCCTG CGACGAGGAC GGCGAGGAGG AGCTTTCTCC AGGGTAGGGC GCGGCGAGGA        480
TCTGGATTTG GTCGTGGAGG CTCCTGATGC AGGCGGCTGC CTCGTGGAGA ACCGACGCGG        540
TGTCCGTCTT GCCGTAGGGG GAAACCAGCT GCTGGAGCGC CGTGATCTTG TCCCCCAGCT        600
TCTGGCTCCT CTTGCACGGT GCCTTGGCAG CTGCTGTATT ATTTCTTGGC TTTGCGCCGC        660
TGCCCTGCAG CCGGCCGTGC GGACCATGCG CCGTCGT                                 697
TypePositionOrientation
ORF 3 - 122: forward
ORF 198 - 311: forward
ORF 696 - 250: reverse
ORF 697 - 278: reverse
Cluster Info:
Contig17200.1 (2 members)
Representative for Uniset
view Blast Searches
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